Macromolecules lab conclusion.

In this lab, we asked the question if macromolecules could be found in an egg cell. With use of the chemicals: Iodine, Sodium Hydroxide, Copper Sulfate, and Sudan III or IV, we were able to find the cells. When we were looking for monosaccharides, we found them in the egg white and yolk. When looking for lipids, we found them in the yolk, egg white, and membrane. We found protein in the yolk, egg white, and membrane. We on only found monosaccharides in the yolk and egg white. When testing for lipids, the chemical we used turned orange when lipids were present. When testing for protein, the chemical turned purple when the protein was present.When testing for polysaccharide, the chemical turned black when polysaccharides were present. The chemical turned green when monosaccharides were present. Since membranes are made out of lipids, it isn't a surprise that we found lipids in the membrane.Protein helps the egg develop, and we found protein in all parts of the egg. Polysaccharides provide energy/food for the egg, so its not too surprising that polysaccharides were found in all parts of the cell. The color changes support my claim because it is the only way to find out where and if the macromolecules are present. One error that might have occurred is contamination. This could lead to false results because of the color change even if there shouldn't be a color change. A second possible error is judgement. Judgement sounds like a silly error; however, it actually is very easy to come across. Sometimes the color may slightly change, so you need to determine if the macromolecules are/aren't present. My first recommendation would be to wash all the tools before changing parts of the egg. My second recommendation would be to get another persons opinion if you are not sure with the color change! The purpose of this lab was to separate the parts of an egg, and find out where certain macromolecules are located. I remember learning about the cell wall in class, and this experiment allowed us to take pieces of the cell wall, and test to see what macromolecules were there. After learning about this, you could use this knowledge with anything related to cells because you have physically touched and looked at one. Now you can use this as a visual whenever thinking about cells.